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The explanation of GSH reduction by glibenclamide treatment may be due to the following possible mechanisms: 1) we found that glibenclamide did not affect the regenerated GSH cycle by the enzyme GR which performs the reduction of GSSG to GSH, and oxidation of NADPH to NAD + 17 , but it reduced GPx levels which performs the reduction of hydrogen peroxide to water, while linking two GSH molecules together by a disulfide bridge to form GSSG